首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   27584篇
  免费   2780篇
  国内免费   3716篇
  2024年   25篇
  2023年   392篇
  2022年   539篇
  2021年   1478篇
  2020年   1111篇
  2019年   1406篇
  2018年   1280篇
  2017年   965篇
  2016年   1229篇
  2015年   1862篇
  2014年   2280篇
  2013年   2365篇
  2012年   2879篇
  2011年   2504篇
  2010年   1635篇
  2009年   1530篇
  2008年   1653篇
  2007年   1499篇
  2006年   1262篇
  2005年   1122篇
  2004年   889篇
  2003年   810篇
  2002年   658篇
  2001年   444篇
  2000年   353篇
  1999年   369篇
  1998年   244篇
  1997年   194篇
  1996年   182篇
  1995年   151篇
  1994年   133篇
  1993年   91篇
  1992年   96篇
  1991年   86篇
  1990年   69篇
  1989年   62篇
  1988年   54篇
  1987年   35篇
  1986年   34篇
  1985年   44篇
  1984年   13篇
  1983年   15篇
  1982年   12篇
  1981年   11篇
  1980年   3篇
  1979年   2篇
  1977年   2篇
  1969年   2篇
  1965年   1篇
  1950年   2篇
排序方式: 共有10000条查询结果,搜索用时 14 毫秒
21.
Acyltransferase (AT)-less type I polyketide synthases (PKSs) produce complex natural products due to the presence of many unique tailoring enzymes. The 3-hydroxy-3-methylglutaryl coenzyme A synthases (HCSs) are responsible for β-alkylation of the growing polyketide intermediates in AT-less type I PKSs. In this study, we discovered a large group of HCSs, closely associated with the characterized and orphan AT-less type I PKSs through in silico genome mining, sequence and genome neighbourhood network analyses. Using HCS-based probes, the survey of 1207 in-house strains and 18 soil samples from different geographic locations revealed the vast diversity of HCS-containing AT-less type I PKSs. The presence of HCSs in many AT-less type I PKSs suggests their co-evolutionary relationship. This study provides a new probe to study the abundance and diversity of AT-less type I PKSs in the environment and microbial strain collections. Our study should inspire future efforts to discover new polyketide natural products from AT-less type I PKSs.  相似文献   
22.
Among the six species of fossil bryozoans described by Xia et al. [Xia, F.S., Zhang, S.G., Wang, Z.Z., 2007. The oldest bryozoans: new evidence from the late Tremadocian (Early Ordovician) of East Yangtze Gorges. Journal of Paleontology 81 (6), 1308–1326] from the Fenghsiang Formation of the Chenjiahe section of Yichang and the Guanzhuangping section, Liujiachang Town of Songzi, the Yangtze Gorges, Nekhorosheviella nodulifera occurs in the pristinus Conodont Subzone (lower Subzone of deltifer Conodont Zone) and the five other species are associated with the conodont Paltodus deltifer deltifer and the graptolites Acanthograptus sinensis and A. erectoramus. Based on the conodonts and graptolites, this bryofauna stratigraphically corresponds to the A. sinensis Graptolite Zone or P. deltifer Conodont Zone. The bryofauna is of Tremadocian Age (Early Ordovician) and represents the oldest bryozoans so far as known.  相似文献   
23.
Plant Molecular Biology Reporter - Global warming threatens many aspects of human life, including a reduction in crop yields, and breeding heat-tolerant crops is a fundamental way to help address...  相似文献   
24.
25.
  相似文献   
26.
Plant Cell, Tissue and Organ Culture (PCTOC) - Tetraploid poplar plants were induced by colchicine from tissue-cultured shoots; the induction efficiency varied with colchicine concentration....  相似文献   
27.
Past event-related potentials (ERPs) research shows that, after exerting effortful emotion inhibition, the neural correlates of performance monitoring (e.g. error-related negativity) were weakened. An undetermined issue is whether all forms of emotion regulation uniformly impair later performance monitoring. The present study compared the cognitive consequences of two emotion regulation strategies, namely suppression and reappraisal. Participants were instructed to suppress their emotions while watching a sad movie, or to adopt a neutral and objective attitude toward the movie, or to just watch the movie carefully. Then after a mood scale, all participants completed an ostensibly unrelated Stroop task, during which ERPs (i.e. error-related negativity (ERN), post-error positivity (Pe) and N450) were obtained. Reappraisal group successfully decreased their sad emotion, relative to the other two groups. Compared with participants in the control group and the reappraisal group, those who suppressed their emotions during the sad movie showed reduced ERN after error commission. Participants in the suppression group also made more errors in incongruent Stroop trials than the other two groups. There were no significant main effects or interactions of group for reaction time, Pe and N450. Results suggest that reappraisal is both more effective and less resource-depleting than suppression.  相似文献   
28.
29.
Demyelination contributes to the functional impairment of irradiation injured spinal cord. One potential therapeutic strategy involves replacing the myelin-forming cells. Here, we asked whether transplantation of Olig2+-GFP+-oligodendrocyte precursor cells (OPCs), which are derived from Olig2-GFP-mouse embryonic stem cells (mESCs), could enhance remyelination and functional recovery after spinal cord irradiation injury. We differentiated Olig2-GFP-mESCs into purified Olig2+-GFP+-OPCs and transplanted them into the rats’ cervical 4–5 dorsal spinal cord level at 4 months after irradiation injury. Eight weeks after transplantation, the Olig2+-GFP+-OPCs survived and integrated into the injured spinal cord. Immunofluorescence analysis showed that the grafted Olig2+-GFP+-OPCs primarily differentiated into adenomatous polyposis coli (APC+) oligodendrocytes (54.6±10.5%). The staining with luxol fast blue, hematoxylin & eosin (LFB/H&E) and electron microscopy demonstrated that the engrafted Olig2+-GFP+-OPCs attenuated the demyelination resulted from the irradiation. More importantly, the recovery of forelimb locomotor function was enhanced in animals receiving grafts of Olig2+-GFP+-OPCs. We concluded that OPC transplantation is a feasible therapy to repair the irradiated lesions in the central nervous system (CNS).  相似文献   
30.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号